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onsdag 21 maj 2014

Tulevaisuudessa kai sitten saa kudosteknologisen uusen ohuensuolen ja voi sitten syödä sitä vehnää.

Käytännössä olisi kyllä  halvempi ratkaisu hydrolysoida ja elintarviketeknologisesti muutenkin käsitellä  viljat niin,  että ne eivät ole suolta tuhoavia antigeenejä. Tässä näyttää kohta olevan   tulossa suoliputkeakin teknisesti. kun vain asian avain löytyy.

Isolation and characterization of human primary enterocytes from small intestine using a novel method.

Artikel, refereegranskad vetenskaplig
Författare Priti Chougule
Gustaf Herlenius
Nidia Maritza Hernandez
Pradeep B Patil
Bo Xu
Suchitra Sumitran-Holgersson
Publicerad i Scandinavian journal of gastroenterology
Volym 47
Nummer/häfte 11
Sidor 1334-43
ISSN 1502-7708
Publiceringsår 2012
Publicerad vid Institutionen för kliniska vetenskaper, sektionen för kirurgi och kirurgisk gastroforskning, Avdelningen för kirurgi
Sidor 1334-43
Länkar dx.doi.org/10.3109/00365521.2012.70...
Ämneskategorier Gastroenterologi


Abstract Cell culture studies of enterocytes are important in many fields. However, there are difficulties in obtaining cell lines from adult human intestine, such as microbial contamination of cultures from the tissue samples, short life span of enterocytes, overgrowth of mesenchymal cells, etc. Various model used to obtain adult intestinal cell lines are very complex requiring use of feeder layer or gel matrices. The aim of this study was to establish a novel method for the simple and reproducible isolation of human enterocytes. Enterocytes were isolated from SI samples (n = 5) obtained from cadaveric donors using a mechanical procedure, and separation with immunomagnetic beads coated with anti-EpCAM antibodies. Light and electron microscopy, flow cytometry and immunocytochemistry techniques were used to characterize the isolated cells. Immunohistochemical staining of normal SB biopsies confirmed that the cell cultures maintained an in vivo phenotype as reflected in cytokeratin expression CK18, CK20 and expression of intestine-specific markers such as sucrase isomaltase and maltase glucoamylase. Furthermore, the cells strongly expressed TLR-5, 6, 7, 8 and 10 and several molecules such as CD40, CD86, CD44, ICAM-1 and HLA-DR which are important in triggering cell-mediated immune responses. This novel technique provides a unique in vitro system to study the biology of enterocytes in normal conditions as well as to study inflammatory processes in various small bowel disorders.
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